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MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI)

产品名称：MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI)
产品型号：A900
产品厂商：Quidel
产品文档：

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简单介绍

MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI)

的详细介绍

产品介绍

品名
MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI)

货号
A900

规格 1 Kit (96 well)

格式
ELISA

样品类型
Plasma/Serum

检测时间
3 hours

样品体积
50 μL (consult the Package Insert for dilution instructions for all sample types)

使用说明
For Research Use Only. Not for use in diagnostic procedures.

产品描述
The MicroVue Complement Multiplex *****s measures the amount of various complement proteins in human plasma or serum specimens. Choose between available off-the-shelf *****s or have a custom kit developed with the specific analytes required (see Ordering Information below for more information).

The included analytes are available in two panels:

Panel 1: Ba, Bb, C2 Intact, C3a, C3d, C4a, C4d, C5a, sC5b-9, Factor D, Factor H, Factor I

Panel 2: C1q, C2 Intact, C3 Intact, C4 Intact, C5 Intact, Factor D, Factor P

Why two panels?
Different complement proteins are found in different amounts withing serum/plasma. Panel 1 analytes require a 1:100 dilution while Panel 2 analytes require a 1:1000 dilution.

For custom panels, only analytes within the same panel can be placed into the same multiplex *****.

兼容性
Certain analytes cannot be combined into the same custom multiplex *****s due to cross-reactivity. Incompatible analytes include:

Panel 1: C3a and C3d

Panel 1: C4a and C4d

背景
The Complement System consists of more than 50 fluid and membrane-bound proteins and protein fragments organized into four primary pathways: the Classical, Alternative, Lectin and Terminal. Through specific activation steps complement proteins mediate a set of activities including inflammation, clearance of immune complexes, disruption of cell membranes, and regulation of the immune response. Deficiencies in the complement cascade can predispose individuals to infection through ineffective opsonization or defects in the ability to lyse invading pathogens. Also, the complement system is associated with many acute and chronic diseases due to abnormalities in its function. Quantitative analysis of different complement proteins and their activation products are of great importance in experimental and clinical medicine. The ability to measure multiple complement proteins in an individual sample constitutes a major step forward in research-based tools. The MicroVue Complement Multiplex can quantitively measure twelve individual complement proteins, providing a comprehensive overview of complement system homeostasis and activation.

分析步骤
Sample volume per well (50 μL)

Sample Dilution

Antigen incubation time (2 hours)

Wash wells (3X)

Detection volume per well (50 μL)

Detection incubation time (1 hour)

Wash wells (3X)

SHRP 1X volume per well (50 μL)

SHRP 1X incubation time (20 minutes)

Wash wells (6X)

Substrate volume per well (50 μL)

Imaging Time (250-300 sec)

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产品介绍
品名	MicroVue Complement Fragment Panel (C4a,Ba, Bb, C3a, C5a, Sc5b-9, FH, FI)
货号	A900
规格	1 Kit (96 well)
格式	ELISA
样品类型	Plasma/Serum
检测时间	3 hours
样品体积	50 μL (consult the Package Insert for dilution instructions for all sample types)
使用说明	For Research Use Only. Not for use in diagnostic procedures.
产品描述	The MicroVue Complement Multiplex ***s measures the amount of various complement proteins in human plasma or serum specimens. Choose between available off-the-shelf *s or have a custom kit developed with the specific analytes required (see Ordering Information below for more information). The included analytes are available in two panels: Panel 1: Ba, Bb, C2 Intact, C3a, C3d, C4a, C4d, C5a, sC5b-9, Factor D, Factor H, Factor I Panel 2: C1q, C2 Intact, C3 Intact, C4 Intact, C5 Intact, Factor D, Factor P Why two panels? Different complement proteins are found in different amounts withing serum/plasma. Panel 1 analytes require a 1:100 dilution while Panel 2 analytes require a 1:1000 dilution. For custom panels, only analytes within the same panel can be placed into the same multiplex ***.
兼容性	Certain analytes cannot be combined into the same custom multiplex *****s due to cross-reactivity. Incompatible analytes include: Panel 1: C3a and C3d Panel 1: C4a and C4d
背景	The Complement System consists of more than 50 fluid and membrane-bound proteins and protein fragments organized into four primary pathways: the Classical, Alternative, Lectin and Terminal. Through specific activation steps complement proteins mediate a set of activities including inflammation, clearance of immune complexes, disruption of cell membranes, and regulation of the immune response. Deficiencies in the complement cascade can predispose individuals to infection through ineffective opsonization or defects in the ability to lyse invading pathogens. Also, the complement system is associated with many acute and chronic diseases due to abnormalities in its function. Quantitative analysis of different complement proteins and their activation products are of great importance in experimental and clinical medicine. The ability to measure multiple complement proteins in an individual sample constitutes a major step forward in research-based tools. The MicroVue Complement Multiplex can quantitively measure twelve individual complement proteins, providing a comprehensive overview of complement system homeostasis and activation.
分析步骤	Sample volume per well (50 μL) Sample Dilution Antigen incubation time (2 hours) Wash wells (3X) Detection volume per well (50 μL) Detection incubation time (1 hour) Wash wells (3X) SHRP 1X volume per well (50 μL) SHRP 1X incubation time (20 minutes) Wash wells (6X) Substrate volume per well (50 μL) Imaging Time (250-300 sec)