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Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag

  • 产品名称：Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag
  • 产品型号：31-1048-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag

的详细介绍

Product Details
Product Name Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody [RM146]
Product Description Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No. 31-1048-00
Clone Name RM146
Product Data Sheet Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
Specificity RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application Western Blot
Immunoprecipitation
Immunocytochemistry
Flow Cytometry
Immunohistochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 ug/mL – 1 ug/mL;
Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry 0.5 ug/mL – 2 ug/mL;
Flow Cytometry: 0.5 ug/mL – 2 ug/mL;
Immunohistochemistry; 0.1 ug/mL-1 ug/mL.
Keywords
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Product Images
Western

Western blot of 293T cells:
(1) untransfected or
 (2) transfected with His-Tag fusion protein X and
(3) E. coli lysate without His-Tag Protein Y
(4) E. coli lysate with His-Tag Protein Y
 Using RevMAb Clone RM146 at 0.2 ug/mL.

Immunoprecipitation

Immunoprecipitation of 293T cells expressing a
His-Tag fusion protein by RevMAb clone RM146,
 was blotted with RM146. (1) Whole lysate control;
 (2) IP by rabbit IgG control; (3) IP by RM146.

Immunohistochemistry

Immunohistochemistry staining of naïve HepG2 cells
(Negative control) using anti-His-Tag antibody, RM146.

Immunohistochemistry

Immunohistochemistry staining of 293T cells expressing
 His-Tag nuclear protein X, using anti-His-Tag antibody,
 RM146.


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