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Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody, Clone RM236; 5′-hmC

  • 产品名称：Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody, Clone RM236; 5′-hmC
  • 产品型号：31-1111-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody, Clone RM236; 5′-hmC

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Product Details
Product Name Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236]
Product Description Rabbit monoclonal to 5-hydroxymethylcytosine (5-hmC); 5'-hmC
Catalog No. 31-1111-00
Clone Name RM236
Product Data Sheet Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody Data Sheet RM236
Specificity RM236 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Immunogen BSA-conjugated 5-hydroxymethylcytosine.
Application Hydroxymethylated DNA immunoprecipitation (hMeDIP)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Dot Blot (DB)
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Dot Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL - 1 ug/mL;
IHC: 0.1 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL- 2 ug/mL;
hMeDIP: 0.2 ug/mL- 2 ug/mL.
References for 5-hydroxymethylcytosine (5-hmC) antibody [RM236] J Reichmann et al. Dual spindle formation in zygotes keeps parental genomes apart in early mammalian embryos,
bioRxiv; 10.1101/198275 (2017). IF; Mouse Read more
.
Product Images
ELISA

ELISA of single stranded DNA using rabbit monoclonal
 anti-5-hmC (clone RM236) antibody. The plate was coated
with streptavidin and then biotinylated single stranded
 unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and
 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution
 of RM236 was used as the primary antibody, and an
 alkaline phosphatase conjugated anti-rabbit IgG as the
 secondary antibody.

Dot

Dot blot of double stranded DNA using rabbit monoclonal
anti-5-hmC (clone RM236) antibody. The membrane was
pre-spotted with 50, 5, and 0.5 ng/dot of double stranded
 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine
(5-mC) DNA, and unmethylated DNA. The pre-spotted
 membrane was then blotted with RM236.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
 paraffin embedded human brain tissue sections, using
 rabbit monoclonal anti-5-hmC (clone RM236) antibody.

Immunocytochemical

Immunocytochemical staining of HeLa cells using
 0.5ug/mL anti-5-hmC antibody (RM236) (red). Actin
filaments was labeled with fluorescein phalloidin
 (green). HeLa cells were fixed with 4% parafor-
 maldehyde and permeabilized with methanol (?20°C)
 before treatment with 2 N HCl for 30 min at 37 °C to
 denature DNA.

Direct

Direct ELISA of mouse brain genomic DNA using
 anti-5-hmC antibody (RM236). The plate was directly
 coated with different concentrations of genomic DNA
isolated from mouse brain tissue. 1 ug/mL or 3 ug/mL
of RM236 was used as the primary antibody, and a
 HRP conjugated anti-rabbit IgG as the secondary
 antibody.

hMeDIP

hMeDIP was performed using anti-5-hmC antibody
 (RM236) at a 10:1 DNA:Ab ratio. 1 ng of unmethylated,
 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine
 (5-hmC) DNA standard (897 bp) was spiked in 1ug of
genomic DNA isolated from HeLa cells as the control.
 Realtime PCR was then performed to determine the
 capture of DNA standard as in % of input.


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