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Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120

  • 产品名称：Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
  • 产品型号：31-1022-02
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120

的详细介绍

Product Details
Product Name Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [RM120]
Product Description Biotinylated Rabbit monoclonal to Human IgG4
Catalog No. 31-1022-02
Clone Name RM120
Product Data Sheet Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
Specificity RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Peptide corresponding to the hinge region of Human IgG4
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Western Blot
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
Keywords
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Product Images
Sandwich

Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
 followed by an AP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
 followed by an AP conjugated streptavidin.

Western

Western Blot of human, mouse, rat, and goat
 IgG shows RM120 reacts to human IgG4, in
 both whole molecule (~150kDa, non-reduced)
 and heavy chain (~50kDa, reduced) forms. No
 cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.

A

A titer ELISA using RM120. The plate was coated
 with different amounts of human IgG4. A serial dilution
 of RM120 was used as the primary antibody. An alkaline
 phosphatase conjugated anti-rabbit IgG as the secondary
 antibody.

Immunohistochemistry

Immunohistochemistry of Human Lymphoid Tissue using
 Anti-Human IgG4 antibody RM120.


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