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Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218

  • 产品名称：Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218
  • 产品型号：31-1094-02
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody, Clone RM218

的详细介绍

Product Details
Product Name Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody [RM218]
Product Description Biotinylated Rabbit monoclonal to Mouse IgG3
Catalog No. 31-1094-02
Clone Name RM218
Product Data Sheet Biotin Anti-Mouse IgG3 Rabbit Monoclonal Antibody Data Sheet RM218
Specificity RM218 reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.
Immunogen Mouse IgG3
Application Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images

ELISA of mouse immunoglobulins shows RM218
 reacts to both mouse IgG3κ and IgG3λ; No cross
reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A titer ELISA of mouse IgG3. The plate was coated
with different amounts of mouse IgG3. A serial
dilution of RM218 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.


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