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Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)

  • 产品名称：Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)
  • 产品型号：31-1055-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)

的详细介绍

Product Details
Product Name Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody [RM166]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)
Catalog No. 31-1055-00
Clone Name RM166
Product Data Sheet Anti-Acetyl-Histone H3 Lys18 Rabbit Monoclonal Antibody Data Sheet RM166
Specificity RM166 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 1 ug/mL-10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
References for Acetyl-Histone H3 (Lys18) antibody [RM166] Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794)
Product Images
RM166

RM166 specifically reacts to Histone H3
 acetylated at Lysine 18 (K18ac). No cross reactivity
 with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
 using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated HeLa.

Sandwich

Sandwich ELISA against acetylated histone H3
at Lys 18 using HeLa whole cell lysate, treated or
 untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
 biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
 detection antibody.

ChIP

ChIP performed on HeLa cells with or without
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
 Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.


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