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Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119

  • 产品名称：Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
  • 产品型号：31-1021-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119

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Product Details
Product Name Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
Product Description Rabbit monoclonal to Human IgG3
Catalog No. 31-1021-00
Clone Name RM119
Product Data Sheet Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
Specificity RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG3
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Keywords
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Product Images
Detection

Detection of human IgG3 in monkey serum.
Sandwich ELISA using RM119 as the capture
 antibody, and biotinylated anti-human light
 chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM119 as the capture antibody
 (100ng/well), and biotinylated anti-human light chains ( κ+ λ)
 antibody RM129 as the detection antibody,
followed
 by an HRP conjugated streptavidin.

ELISA

ELISA showing RM119 reacts only to human IgG3,
and not to any other IgG subclasses (IgG1, IgG2,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.

A

A titer ELISA using RM119. The plate was coated
with different amounts of human IgG3. A serial dilution
 of RM119 was used as the primary antibody, followed
 by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.

Immunohistochemical staining of formalin fixed
 and paraffin embedded human lymphoid tissue
 section using anti-human IgG3 rabbit monoclonal
antibody (Clone RM119).

ELISA

ELISA showing RM119 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM119 and a monkey IgG binding
 antibody (positive control) was used as the detection
antibody.

Immunohistochemical staining of FFPE Tonsil tissue
 sections using Anti-Human IgG3 antibody RM119
 at a 1:2000 dilution.

Image courtesy of Shaomin Hu, MD, PhD,
Department of Anatomic and Clinical Pathology.
Montefiore Medical Center
 Albert Einstein College of Medicine.
 Bronx, New York, USA


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