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Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231

  • 产品名称：Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231
  • 产品型号：31-1110-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231

的详细介绍

Product Details
Product Name Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody [RM231]
Product Description Rabbit monoclonal to 5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, 5'-mC
Catalog No. 31-1110-00
Clone Name RM231
Product Data Sheet Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody Data Sheet RM231
Specificity RM231 reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Immunogen BSA-conjugated 5-methylcytosine.
Application Methylated DNA immunoprecipitation (MeDIP)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Dot Blot (DB)
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Dot Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.1 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
MeDIP: 0.2 ug/mL- 2 ug/mL.
Keywords
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Product Images
ELISA

ELISA of single stranded DNA using rabbit monoclonal
 anti-5-mC (clone RM231) antibody. The plate was coated
with streptavidin and then biotinylated single stranded
 unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and
 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution
 of RM231 was used as the primary antibody, and an
 alkaline phosphatase conjugated anti-rabbit IgG as the
 secondary antibody.

Dot

Dot blot of double stranded DNA using rabbit monoclonal
anti-5-mC (clone RM231) antibody. The membrane was
pre-spotted with 50, 5, and 0.5 ng/dot of double stranded
 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine
(5-mC) DNA, and unmethylated DNA. The pre-spotted
 membrane was then blotted with RM231.

Immunocytochemical

Immunocytochemical staining of HeLa cells using rabbit
monoclonal anti-5-mC (clone RM231) antibody (red).
 Actin filaments have been labeled with fluorescein
 phalloidin (green), and nuclei stained with DAPI (blue).

Direct

Direct ELISA of HeLa cell genomic DNA using anti-5-mC
 antibody (RM231). The plate was directly coated with
 different concentrations of genomic DNA isolated from
HeLa cells. 1 ug/mL or 3 ug/mL of RM231 was used as
 the primary antibody, and a HRP conjugated anti-rabbit IgG
 as the secondary antibody.

MeDIP

MeDIP was performed using anti-5-mC antibody
 (RM231) at a 2:1 DNA:Ab ratio. 1 ng of unmethylated,
5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine
 (5-hmC) DNA standard (897 bp) was spiked in 1ug of
 genomic DNA isolated from HeLa cells as the control.
Realtime PCR was then performed to determine the
 capture of DNA standard as in % of input.


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