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Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

  • 产品名称：Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111
  • 产品型号：32-1011-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

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Product Details
Product Name Anti-Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
Product Description Rabbit monoclonal to Phosphotyrosine
Catalog No. 32-1011-00
Clone Name RM111
Product Data Sheet Anti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111
Specificity RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
Immunogen Phosphotyrosine-BSA conjugate
Application Western Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity All Species
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot (WB): 1:1000 -1:5000 dilution;
Immunoprecipitation (IP): 1:200-1:1000 dilution;
Chromatin IP (ChIP): 1:200-1:1000 dilution;
Immunocytochemistry (ICC): 1:200-1:500 dilution;
Immunohistochemistry (IHC): 1:200-1:500 dilution.
Keywords
References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111] Sun et al., Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity, Neuron (2016), http://dx.doi.org/
10.1016/j.neuron.2016.08.033. IHC; Mouse Read more (PubMed: 27641496)
Product Images
Immunocytochemistry

Immunocytochemistry of serum-starved A431
 cells nontreated or treated with EGF, using
RevMAb Clone RM111 at 1/500 dilution (foll-
owed by a PE conjugated secondary antibody,
red) and DAPI (blue).

Western

Western blot of serum-starved A431 cells non-
treated or treated with EGF, using RevMAb
Clone RM111 at 1/5000 dilution. The blot was
 exposed to the film at different time points.

Immunoprecipitation

Immunoprecipitation of EGF-treated A431 cells
 by RevMAb Cone RM111 at 1/1000 dilution, was
 blotted with an anti-EGFR rabbit monoclonal antibody.
 (1) Whole lysate control; (2) IP by rabbit IgG control;
(3) IP by Clone RM111.

Luminex

Luminex analysis of Clone RM111’s reaction
 against phosphorylated or nonphosphorylated
Serine, Threonine, and Tyrosine. RevMAb Clone
 RM111 reacts only to phosphorylated Tyrosine.


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