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Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody, Clone RM107

  • 产品名称:Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody, Clone RM107
  • 产品型号:31-1003-00
  • 产品厂商:RevMAb
  • 产品文档:
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简单介绍

Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody, Clone RM107

的详细介绍

Product Details
Product Name Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [RM107]
Product Description Rabbit monoclonal to Mouse IgG2a Kappa
Catalog No. 31-1003-00
Clone Name RM107
Product Data Sheet Anti-Mouse IgG2a Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM107
Specificity RM107 reacts to the Fab region of mouse IgG2aκ. No cross reactivity with mouse IgG2aΚ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM107 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgG
Application Western Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2aκ (20 ng/lane), using 0.2ug/mL
of RevMAb clone RM107. This antibody only
reacts to nonreduced Mouse IgG2aκ.

ELISA

ELISA of mouse immunoglobulins shows RM107
reacts to the Fab region of mouse IgG2aκ; no cross
reactivity with IgG2aκ, IgG1, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG2aκ. The plate was
coated with different amounts of mouse IgG2aκ.
A serial dilution of RM107 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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