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Anti-cGMP Rabbit Monoclonal Antibody, Clone RM467

  • 产品名称：Anti-cGMP Rabbit Monoclonal Antibody, Clone RM467
  • 产品型号：31-1359-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-cGMP Rabbit Monoclonal Antibody, Clone RM467

的详细介绍
Product Details
Product Name Anti-cGMP Rabbit Monoclonal Antibody [RM467]
Product Description Rabbit monoclonal to cGMP (Cyclic Guanosine Monophosphate)
Catalog No. 31-1359-00
Clone Name RM467
Product Data Sheet Anti-cGMP Rabbit Monoclonal Antibody Data Sheet RM467
Specificity RM467 reacts to cGMP (Cyclic Guanosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
Immunogen KLH-conjugated cGMP
Application ELISA
Conjugate None
Intended Use Research Use Only
Related Product Antigen for Competitive Assay 71-1359-00 - cGMP-HRP
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50ug
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.05 ug/mL – 0.5 ug/mL
Keywords
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Product Images

Competitive ELISA of cGMP using anti-cGMP (RM467). The 96-well plate was coated
with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cGMP (RM467) (50 µl/well)
was added and incubated. After wash and block, different concentrations of cGMP (25 µl/well)
were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cGMP (71-1359-00).
TMB was used to develop the color after incubation and wash.

Competitive ELISA showing the specificity of anti-cGMP (RM467). The 96-well
plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml
of anti-cGMP (RM467)(50 µl/well) was added and incubated. After wash
and block, cGMP and other cyclic nucleotides or nucleoside phosphates
(25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP
conjugated cGMP (71-1359-00). TMB was used to develop
the color after incubation and wash.

Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with
1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cGMP (RM467)
(50 µl/well) was added and incubated. After wash and block, HeLa cell lysate
(~105cell/mL) or control (lysate buffer only) (25 µl/well) were added along with
25 µl/well of 1/5,000 diluted HRP conjugated cGMP (71-1359-00).
TMB was used to develop the color after incubation and wash.

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