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Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161; H3K9ac, Histone H3 (K9 acetylation); ChIP Validated

  • 产品名称：Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161; H3K9ac, Histone H3 (K9 acetylation); ChIP Validated
  • 产品型号：31-1054-00
  • 产品厂商：RevMAb
  • 产品文档：
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简单介绍

Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM161; H3K9ac, Histone H3 (K9 acetylation); ChIP Validated

的详细介绍

Product Details
Product Name Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM161]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation)
Catalog No. 31-1054-00
Clone Name RM161
Product Data Sheet Anti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161
Specificity RM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 0.1 ug/mL – 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
References for Acetyl-Histone H3 (Lys9) antibody [RM161] Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794)
Product Images
RM161

RM161 specifically reacts to Histone H3 acetylated
 at Lysine 9 (K9ac). No cross reactivity with acetyl-
ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18
 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
 Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79
 (K79ac), or Lysine 122 (K122) in histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
 using RM161 at 0.25 ug/mL, showed a band of
histone H3 acetylated at Lysine 9 in treated HeLa.

Sandwich

Sandwich ELISA against acetylated histone H3 at Lys 9
 using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as
the capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.

ChIP

ChIP performed on HeLa cells using H3K9ac antibody (RM161,
5ug). Real-time PCR was performed using primers specific to
 the gene indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys9)
Rabbit mAb RM161 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
anti-Acetyl-Histone H3 (Lys9) antibody, RM161.


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